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constitutive promoter ef1α  (Addgene inc)


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    Addgene inc constitutive promoter ef1α
    Constitutive Promoter Ef1α, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constitutive+promoter+ef1%CE%B1/pOTTC407+-+pAAV+EF1a+eGFP+(Plasmid+%2360058)/pmc12802846-169-24-27
    Average 94 stars, based on 18 article reviews
    constitutive promoter ef1α - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: A programmable genetic platform for engineering noninvasive biosensors.
    Article Snippet: .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible minimal CMV promoter (Addgene, #26431). ..

    Article Title: A programmable genetic platform for engineering noninvasive biosensors
    Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible minimal CMV promoter (Addgene, #26431). ..

    Clone Assay:

    Article Title: A programmable genetic platform for engineering noninvasive biosensors.
    Article Snippet: .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible minimal CMV promoter (Addgene, #26431). ..

    Article Title: A programmable genetic platform for engineering noninvasive biosensors
    Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible minimal CMV promoter (Addgene, #26431). ..

    Plasmid Preparation:

    Article Title: A programmable genetic platform for engineering noninvasive biosensors.
    Article Snippet: .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible minimal CMV promoter (Addgene, #26431). ..

    Article Title: A programmable genetic platform for engineering noninvasive biosensors
    Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible minimal CMV promoter (Addgene, #26431). ..

    Control:

    Article Title: A programmable genetic platform for engineering noninvasive biosensors.
    Article Snippet: .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible minimal CMV promoter (Addgene, #26431). ..

    Article Title: A programmable genetic platform for engineering noninvasive biosensors
    Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible minimal CMV promoter (Addgene, #26431). ..



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    Image Search Results


    Levels of pro-IL-1β are reduced in HCMV-positive cells. (A) Immunofluorescence microscopy showing HCMV IE expression in THP-1 cells infected at the indicated MOI overnight. Yellow markers indicate DAPI-stained cells that lack detectable nuclear IE protein. (B) Immunoblot assay for GSDMD in ΔHUMCYC and ΔGSDMD THP-1 cells. (C, D) Intracellular staining for IL-1β in ΔGSDMD EF1α-pro-IL-1β THP-1 cells either mock infected or infected with HCMV-TB40/E (MOI of 3) for 18 h (C) and gated for virally encoded GFP (D). GFP-positive (GFP+) population depicts the number of HCMV-infected cells in the sample. The bar graphs show the mean fluorescence intensities (MFI) for IL-1β staining. The insets show IL-1β staining in the indicated populations. Values are averages ± SEM from two biological replicates. Student’s t test was performed. *, P < 0.05; **, P < 0.01.

    Journal: mBio

    Article Title: Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome

    doi: 10.1128/mBio.02510-18

    Figure Lengend Snippet: Levels of pro-IL-1β are reduced in HCMV-positive cells. (A) Immunofluorescence microscopy showing HCMV IE expression in THP-1 cells infected at the indicated MOI overnight. Yellow markers indicate DAPI-stained cells that lack detectable nuclear IE protein. (B) Immunoblot assay for GSDMD in ΔHUMCYC and ΔGSDMD THP-1 cells. (C, D) Intracellular staining for IL-1β in ΔGSDMD EF1α-pro-IL-1β THP-1 cells either mock infected or infected with HCMV-TB40/E (MOI of 3) for 18 h (C) and gated for virally encoded GFP (D). GFP-positive (GFP+) population depicts the number of HCMV-infected cells in the sample. The bar graphs show the mean fluorescence intensities (MFI) for IL-1β staining. The insets show IL-1β staining in the indicated populations. Values are averages ± SEM from two biological replicates. Student’s t test was performed. *, P < 0.05; **, P < 0.01.

    Article Snippet: Myc-tagged pro-IL-1β or pro-IL-18 was cloned into and expressed from a lentiviral vector containing the constitutive EF1α promoter (pLVX - EF1alpha - IRES; Clontech).

    Techniques: Immunofluorescence, Microscopy, Expressing, Infection, Staining, Western Blot, Fluorescence